rabbit anti βactin  (Proteintech)

Journal: Animals : an open access journal from MDPI

Article Title: Antiviral Activity of 1-Deoxynojirimycin Extracts of Mulberry Leaves Against Porcine Epidemic Diarrhea Virus.

doi: 10.3390/ani15091207

Figure Lengend Snippet: Figure 2. DNJ inhibits PEDV infection in Vero-E6 cells. (A) The overall design of Vero E6 cells infected and treated with DNJ (25 µM, 50 µM, 100 µM). The green line indicates drug treatment, and the orange line indicates PEDV infection. (B) Samples were collected at 6, 12, and 24 h post-infection with SQ2014 (MOI = 0.1), and the protein levels of PEDV-N and actin were detected by Western blot. (C) After 12 h of infection, the protein samples with different concentrations of DNJ were detected by Western blot. (D) Quantitative detection of PEDV S and N mRNA level by qRT-PCR. (E) The cells were collected 12 h after infection, and the virus titer of SQ2014 was detected by PFU. The means and standard deviations of three independent experimental replicates are shown. ** p < 0.01; *** p < 0.001.

Article Snippet: The PEDV strain SQ2014 (GenBank accession No. KP728470) and Rabbit anti-PEDV N protein polyclonal antibodies were kindly provided by Professor Qian Yingjuan (Nanjing Agricultural University) [22]. βActin Rabbit polyclonal antibodies were purchased from Proteintech (20536-1-AP, Nanjing, China).

Techniques: Infection, Western Blot, Quantitative RT-PCR, Virus

Journal: Animals : an open access journal from MDPI

Article Title: Antiviral Activity of 1-Deoxynojirimycin Extracts of Mulberry Leaves Against Porcine Epidemic Diarrhea Virus.

doi: 10.3390/ani15091207

Figure Lengend Snippet: Figure 3. The inhibition stage of DNJ on PEDV. (A) This section describes the comprehensive approach for infecting Vero-E6 cells and administering DNJ at a concentration of 100 µM. The treatment procedure is divided into three separate phases: pre-treatment, co-treatment, and post- treatment. (B) In accordance with the treatment protocol depicted in (A), Western blot analysis was conducted to evaluate the protein levels of both the virus and actin. (C) DNJ directly targets PEDV SQ2014 experimental results.

Article Snippet: The PEDV strain SQ2014 (GenBank accession No. KP728470) and Rabbit anti-PEDV N protein polyclonal antibodies were kindly provided by Professor Qian Yingjuan (Nanjing Agricultural University) [22]. βActin Rabbit polyclonal antibodies were purchased from Proteintech (20536-1-AP, Nanjing, China).

Techniques: Inhibition, Concentration Assay, Western Blot, Virus

Journal: Cancers

Article Title: Partial Hepatectomy Promotes the Development of KRASG12V-Induced Hepatocellular Carcinoma in Zebrafish

doi: 10.3390/cancers16101793

Figure Lengend Snippet: The effect of s100a1 knockdown on the development of kras G12V -induced HCC in zebrafish larvae. ( A ) Experiment design of s100a1 knockdown and oncogene induction in Lipan larvae and kras+ larvae. ( B ) Western blot of S100A1with the total protein extracted from MO-injected zebrafish embryos at 24 hpf. β-actin served as a loading control. Each protein sample was pooled from 15 zebrafish embryos. The quantification of the intensity of S100A1normalized by the intensity of β-actin was at the lower panel. The uncropped image of blots is shown in . ( C ) Fluorescence images of morpholino-treated Lipan and kras+ larvae after 48 h of Dox treatment. ( D ) Measurement of liver size based on the fluorescence in ( B ) ( n ≥ 8). Circles, squares, and triangles indicate values of individual samples ( E ) Expression of sox5, sox9a, and sox9b in the kras+ livers at 24 h after PH/sham surgery as determined by RT-qPCR ( n = 3). Scale Bar = 200 μm. ns p > 0.05, * p ≤ 0.05, ** p ≤ 0.01.

Article Snippet: The effect of MO- s100a1 -ATG on S100A1 expression was validated in 1-dpf zebrafish embryos with western blotting using the Rabbit-anti-βactin antibody (bs-0061R, Bioss Antibodies, Woburn, MA, USA) (1:5000) and the Rabbit-anti-S100A1 antibody (50266-RP02; Sino Biological, Beijing, China) (1:1000).

Techniques: Knockdown, Western Blot, Injection, Control, Fluorescence, Expressing, Quantitative RT-PCR